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3 September 2015 Deciphering the internal complexity of living cells with quantitative phase microscopy: a multiscale approach
Cristina E. Martinez-Torres, Bastien Laperrousaz, Lotfi Berguiga, Elise Boyer-Provera, Juan Elezgaray, Franck E. Nicolini M.D., Véronique Maguer-Satta, Alain Arneodo, Françoise Argoul
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Journal of Biomedical Optics, Vol. 20, Issue 9, 096005 (September 2015). https://doi.org/10.1117/1.JBO.20.9.096005
Abstract
The distribution of refractive indices (RIs) of a living cell contributes in a nonintuitive manner to its optical phase image and quite rarely can be inverted to recover its internal structure. The interpretation of the quantitative phase images of living cells remains a difficult task because (1) we still have very little knowledge on the impact of its internal macromolecular complexes on the local RI and (2) phase changes produced by light propagation through the sample are mixed with diffraction effects by the internal cell bodies. We propose to implement a two-dimensional wavelet-based contour chain detection method to distinguish internal boundaries based on their greatest optical path difference gradients. These contour chains correspond to the highest image phase contrast and follow the local RI inhomogeneities linked to the intracellular structural intricacy. Their statistics and spatial distribution are the morphological indicators suited for comparing cells of different origins and/or to follow their transformation in pathologic situations. We use this method to compare nonadherent blood cells from primary and laboratory culture origins and to assess the internal transformation of hematopoietic stem cells by the transduction of the BCR-ABL oncogene responsible for the chronic myelogenous leukemia.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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Cristina E. Martinez-Torres, Bastien Laperrousaz, Lotfi Berguiga, Elise Boyer-Provera, Juan Elezgaray, Franck E. Nicolini M.D., Véronique Maguer-Satta, Alain Arneodo, and Françoise Argoul "Deciphering the internal complexity of living cells with quantitative phase microscopy: a multiscale approach," Journal of Biomedical Optics 20(9), 096005 (3 September 2015). https://doi.org/10.1117/1.JBO.20.9.096005
Published: 3 September 2015
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Cited by 22 scholarly publications.
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KEYWORDS
Microscopy
Spherical lenses
Optical spheres
Blood
Wavelets
Diffraction
Image restoration
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