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16 December 2013 mPlum-IFP 1.4 fluorescent fusion protein may display Förster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging
Liang-Ting Lin, Bo-Sheng Wang, Jyh-Cheng Chen, Chi-Hsien Liu, Chien Chou, Shu-Jun Chiu, Wen-Yi Chang, Ren-Shyan Liu, C. Allen Chang, Yi-Jang Lee
Author Affiliations +
Funded by: National Science Council of Taiwan, National Science Council of Taiwan, China, Taiwan Mouse Clinic
Journal of Biomedical Optics, Vol. 18, Issue 12, 126013 (December 2013). https://doi.org/10.1117/1.JBO.18.12.126013
Abstract
Bacteriophytochrome infrared fluorescent protein (IFP) has a long emission wavelength that is appropriate for detecting pathophysiological effects via near-infrared (NIR) based imaging. However, the brightness and photostability of IFP are suboptimal, although an exogenous supply of biliverdin (BV) IXα is able to enhance these properties. In this study, we fused a far red mPlum fluorescent protein to IFP 1.4 via a linker deoxyribonucleic acid (DNA) sequence encoding eight amino acids. The brightness of mPlum-IFP 1.4 fusion protein at the IFP emission channel was comparable to that of native IFP 1.4 protein when fusion protein and IFP 1.4 were excited by 543 and 633 nm using confocal microscopy, respectively. Visualization of IFP 1.4 fluorescence by excitation of mPlum in mPlum-IFP 1.4 fusion protein is likely to be associated with Förster resonance energy transfer (FRET). The FRET phenomenon was also predicted by acceptor photobleaching using confocal microscopy. Furthermore, the expression of mPlum-IFP 1.4 fusion protein could be detected in cell culture and in xenograft tumors in the absence of BV using in vivo imaging system, although the BV was still essential for detecting native IFP 1.4. Therefore, this innovativefluorescent fusion protein would be useful for NIR-based imaging in vitro and in vivo.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Liang-Ting Lin, Bo-Sheng Wang, Jyh-Cheng Chen, Chi-Hsien Liu, Chien Chou, Shu-Jun Chiu, Wen-Yi Chang, Ren-Shyan Liu, C. Allen Chang, and Yi-Jang Lee "mPlum-IFP 1.4 fluorescent fusion protein may display Förster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging," Journal of Biomedical Optics 18(12), 126013 (16 December 2013). https://doi.org/10.1117/1.JBO.18.12.126013
Published: 16 December 2013
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Cited by 5 scholarly publications and 2 patents.
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KEYWORDS
Proteins
Fluorescent proteins
Fluorescence resonance energy transfer
In vivo imaging
Signal detection
Infrared radiation
Resonance energy transfer
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