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23 July 2013 Deep-tissue photoacoustic tomography of Förster resonance energy transfer
Yu Wang, Jun Xia, Lihong V. Wang
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Journal of Biomedical Optics, Vol. 18, Issue 10, 101316 (July 2013). https://doi.org/10.1117/1.JBO.18.10.101316
Abstract
Förster resonance energy transfer (FRET) is a distance-dependent process that transfers excited state energy from a donor molecule to an acceptor molecule without the emission of a photon. The FRET rate is determined by the proximity between the donor and the acceptor molecules; it becomes significant only when the proximity is within several nanometers. Therefore, FRET has been applied to visualize interactions and conformational changes of biomolecules, such as proteins, lipids, and nucleic acids that cannot be resolved by optical microscopy. Here, we report photoacoustic tomography of FRET efficiency at a 1-cm depth in chicken breast tissue, whereas conventional high-resolution fluorescence imaging is limited to <0.1  cm . Photoacoustic tomography is expected to facilitate the examination of FRET phenomena in living organisms.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Yu Wang, Jun Xia, and Lihong V. Wang "Deep-tissue photoacoustic tomography of Förster resonance energy transfer," Journal of Biomedical Optics 18(10), 101316 (23 July 2013). https://doi.org/10.1117/1.JBO.18.10.101316
Published: 23 July 2013
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Cited by 15 scholarly publications.
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KEYWORDS
Fluorescence resonance energy transfer
Photoacoustic tomography
Luminescence
Photoacoustic spectroscopy
Tissue optics
Resonance energy transfer
Rhodamine
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