In vivo validation of quantitative frequency domain fluorescence tomography

Yuting Lin; Michael T. Ghijsen; Orhan Nalcioglu; Gultekin Gulsen

doi:10.1117/1.JBO.17.12.126021

18 December 2012 In vivo validation of quantitative frequency domain fluorescence tomography

Yuting Lin, Michael T. Ghijsen, Orhan Nalcioglu, Gultekin Gulsen

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Journal of Biomedical Optics, Vol. 17, Issue 12, 126021 (December 2012). https://doi.org/10.1117/1.JBO.17.12.126021

Abstract

We have developed a hybrid frequency domain fluorescence tomography and magnetic resonance imaging system (MRI) for small animal imaging. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration and lifetime images using a multi-modality approach. In vivo experiments are undertaken to evaluate the system. We compare the recovered fluorescence parameters with and without MRI structural a priori information. In addition, we compare two optical background heterogeneity correction methods: Born normalization and utilizing diffuse optical tomography (DOT) functional a priori information. The results show that the concentration and lifetime of a 4.2-mm diameter indocyanine green inclusion located 15 mm deep inside a rat can be recovered with less than a 5% error when functional a priori information from DOT and structural a priori information from MRI are utilized.

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Yuting Lin, Michael T. Ghijsen, Orhan Nalcioglu, and Gultekin Gulsen "In vivo validation of quantitative frequency domain fluorescence tomography," Journal of Biomedical Optics 17(12), 126021 (18 December 2012). https://doi.org/10.1117/1.JBO.17.12.126021

Published: 18 December 2012

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