Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography

Hrebesh M. Subhash; Viviana Davila; Hai Sun; Anh T. Nguyen-Huyn; Alfred L. Nuttall; Ruikang K. Wang

doi:10.1117/1.3456554

1 May 2010 Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography

Hrebesh M. Subhash, Viviana Davila, Hai Sun, Anh T. Nguyen-Huyn, Alfred L. Nuttall, Ruikang K. Wang

Author Affiliations +

Journal of Biomedical Optics, Vol. 15, Issue 3, 036024 (May 2010). https://doi.org/10.1117/1.3456554

Abstract

There is considerable interest in developing new methods for in vivo imaging of the complex anatomy of the mammalian cochlea for clinical as well as fundamental studies. In this study, we explored, the feasibility of spectral domain optical coherence tomography (SD-OCT) for 3-D in vivo imaging of the cochlea in mice. The SD-OCT system employed in this study used a broadband light source centered at 1300 nm, and the imaging speed of the system was 47,000 A-scans per second using the InGaAs camera. The system was capable of providing fully processed, high-resolution B-scan images [512 (axial)×128 (lateral) pixels] at 280 frames per sec. The 3-D imaging acquisition time for a whole cochlea was ~0.45 sec. The traditional SD-OCT structural imaging algorithm was used to reconstruct 3-D cochlear morphology. We demonstrated that SD-OCT can be successfully used for in vivo imaging of important morphological features within the mouse cochlea, such as the otic capsule and structures within, including Reissner's membrane, the basilar membrane, tectorial membrane, organ of Corti, and modiolus of the apical and middle turns.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Hrebesh M. Subhash, Viviana Davila, Hai Sun, Anh T. Nguyen-Huyn, Alfred L. Nuttall, and Ruikang K. Wang "Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography," Journal of Biomedical Optics 15(3), 036024 (1 May 2010). https://doi.org/10.1117/1.3456554

Published: 1 May 2010

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY