In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography

Anne Koeing; Lionel Herve; Véronique Josserand; Michel Berger; Jérôme Boutet; Anabela Da Silva; Jean-Marc Dinten; Philippe Peltie; Jean-Luc Coll; Philippe Rizo

doi:10.1117/1.2884505

1 January 2008 In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography

Anne Koeing, Lionel Herve, Véronique Josserand, Michel Berger, Jérôme Boutet, Anabela Da Silva, Jean-Marc Dinten, Philippe Peltie, Jean-Luc Coll, Philippe Rizo

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Journal of Biomedical Optics, Vol. 13, Issue 1, 011008 (January 2008). https://doi.org/10.1117/1.2884505

Abstract

We present in vivo experiments conducted with a new fluorescence diffuse optical tomographic (fDOT) system on cancerous mice bearing mammary murine tumors. We first briefly present this new system that has been developed and its associated reconstruction method. Its main specificity is its ability to reconstruct the fluorescence yield even in heterogeneous and highly attenuating body regions such as lungs and to enable mouse inspection without immersion in optical index matching liquid (Intralipid and ink). Some phantom experiments validate the performance of this new system for heterogeneous media inspection. Its use for a mice study is then related. It consists in the follow-up of the lungs at different stages of tumor development after injection of RAFT-(cRGD)₄-Alexa700. As expected, the reconstructed fluorescence increases along with the tumor stage. These results validate the use of our system for biological studies of small animals.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Anne Koeing, Lionel Herve, Véronique Josserand, Michel Berger, Jérôme Boutet, Anabela Da Silva, Jean-Marc Dinten, Philippe Peltie, Jean-Luc Coll, and Philippe Rizo "In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography," Journal of Biomedical Optics 13(1), 011008 (1 January 2008). https://doi.org/10.1117/1.2884505

Published: 1 January 2008

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