Nonlinear optical potentiometric dyes optimized for imaging with 1064-nm light

Thomas Z. Teisseyre; Andrew C. Millard; Ping Yan; Joseph P. Wuskell; Mei-de Wei; Aaron Lewis; Leslie M. Loew

doi:10.1117/1.2772276

1 July 2007 Nonlinear optical potentiometric dyes optimized for imaging with 1064-nm light

Thomas Z. Teisseyre, Andrew C. Millard, Ping Yan, Joseph P. Wuskell, Mei-de Wei, Aaron Lewis, Leslie M. Loew

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Journal of Biomedical Optics, Vol. 12, Issue 4, 044001 (July 2007). https://doi.org/10.1117/1.2772276

Abstract

Nonlinear optical phenomena, such as two-photon fluorescence (2PF) and second harmonic generation (SHG), in combination with voltage sensitive dyes, can be used to acquire high-resolution spatio temporal maps of electrical activity in excitable cells and tissue. Developments in 1064-nm fiber laser technology have simplified the generation of high-intensity, long-wavelength, femtosecond light pulses, capable of penetrating deep into tissue.To merge these two advances requires the design and synthesis of new dyes that are optimized for longer wavelengths and that produce fast and sensitive responses to membrane potential changes. In this work, we have systematically screened a series of new dyes with varying chromophores and sidechains that anchor them in cell membranes. We discovered several dyes that could potentially be used for in vivo measurements of cellular electrical activity because of their rapid and sensitive responses to membrane potential. Some of these dyes show optimal activity for SHG; others for 2PF. This regulated approach to dye screening also allows significant insight into the molecular mechanisms behind both SHG and 2PF. In particular, the differing patterns of sensitivity and kinetics for these two nonlinear optical modalities indicate that their voltage sensitivity originates from differing mechanisms.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Thomas Z. Teisseyre, Andrew C. Millard, Ping Yan, Joseph P. Wuskell, Mei-de Wei, Aaron Lewis, and Leslie M. Loew "Nonlinear optical potentiometric dyes optimized for imaging with 1064-nm light," Journal of Biomedical Optics 12(4), 044001 (1 July 2007). https://doi.org/10.1117/1.2772276

Published: 1 July 2007

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Cited by 27 scholarly publications and 3 patents.

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KEYWORDS

Second-harmonic generation

Chromophores

Signal detection

Luminescence

Head

Fiber lasers

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