Immunofluorescence detection with quantum dot bioconjugates for hepatoma in vivo

Xuefeng Yu; Liangdong Chen; Kaiyang Li; Yan Li; Si Xiao; Xuan Luo; Liu Jia; Li Zhou; Yuliang Deng; DaiWen Pang; Ququan Wang

doi:10.1117/1.2437744

1 January 2007 Immunofluorescence detection with quantum dot bioconjugates for hepatoma in vivo

Xuefeng Yu, Liangdong Chen, Kaiyang Li, Yan Li, Si Xiao, Xuan Luo, Liu Jia, Li Zhou, Yuliang Deng, DaiWen Pang, Ququan Wang

Author Affiliations +

Journal of Biomedical Optics, Vol. 12, Issue 1, 014008 (January 2007). https://doi.org/10.1117/1.2437744

Abstract

The use of highly specific and highly sensitive immunofluorescent probes is a promising approach for biomedical imaging in living tissue. We focus on immunofluorescence with quantum dot bioconjugates for hepatoma detection in vivo. We synthesized specific immunofluorescent probes by linking quantum dots to AFP (alpha-fetoprotein) antibody for specific binding AFP—an important marker for hepatocellular carcinoma cell lines. In in vivo studies, the characteristic quantum dot (QD) fluorescent property is exhibited by the QDs-Anti-AFP probes in tumor and they demonstrate active tumor targeting and spectroscopic hepatoma imaging with an integrated fluorescence imaging system. We investigate the inhomogeneous distribution of the QDs-Anti-AFP probes in tumor by using a site-by-site measurement method to test their ability for distribution studies of cancer cells. These results demonstrate the practicality of QD bioconjugates as attractive fluorescent probes for biomedical detection.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Xuefeng Yu, Liangdong Chen, Kaiyang Li, Yan Li, Si Xiao, Xuan Luo, Liu Jia, Li Zhou, Yuliang Deng, DaiWen Pang, and Ququan Wang "Immunofluorescence detection with quantum dot bioconjugates for hepatoma in vivo," Journal of Biomedical Optics 12(1), 014008 (1 January 2007). https://doi.org/10.1117/1.2437744

Published: 1 January 2007

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KEYWORDS

Tumors

Luminescence

Cancer

In vivo imaging

Quantum dots

Imaging systems

Target detection

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