CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 11 > Issue 2 > Article
Open Access
1 March 2006 Photosensitizer delivery to vulnerable atherosclerotic plaque: comparison of macrophage-targeted conjugate versus free chlorine(e6)
Ahmed Tawakol, Ana P. Castano, Florencia Anatelli, Gregory Bashian, Jeremy Stern, Touqir Zahra, Faten Gad, Stephanie Chirico, Atosa Ahmadi, Alan J. Fischman, James E. Muller, Michael R. Hamblin
Author Affiliations +
Journal of Biomedical Optics, Vol. 11, Issue 2, 021008 (March 2006). https://doi.org/10.1117/1.2186039
Abstract
We have previously shown that a conjugate (MA-ce6) between maleylated serum albumin and the photosensitizer chlorin(e6) (ce6) is targeted in vitro to macrophages via class A scavenger receptors. We now report on the ability of this conjugate to localize in macrophage-rich atherosclerotic plaques in vivo. Both the conjugate and the free photosensitizer ce6 are studied after injection into New Zealand White rabbits that are rendered atherosclerotic by a combination of aortic endothelial injury and cholesterol feeding into normal rabbits. Rabbits are sacrificed at 6 and 24 h after injection and intravascular fluorescence spectroscopy is carried out by fiber-based fluorimetry in intact blood-filled arteries. Surface spectrofluorimetry of numbered excised aortic segments together with injured and normal iliac arteries is carried out, and quantified ce6 content by subsequent extraction and quantitative fluorescence determination of the arterial segments and also of nontarget organs. There is good agreement between the various techniques for quantifying ce6 localization, and high contrast between arteries from atherosclerotic and normal rabbits is obtained. Fluorescence correlates with the highest burden of plaque in the aorta and the injured iliac artery. The highest accumulation in plaques is obtained using MA-ce6 at 24 h. Free ce6 gives better accumulation at 6 h compared to 24 h. The liver, spleen, lung, and gall bladder have the highest uptake in nontarget organs. Macrophage-targeted photosensitizer conjugates may have applications in both detecting and treating inflamed vulnerable plaque.
©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation Download Citation
Ahmed Tawakol, Ana P. Castano, Florencia Anatelli, Gregory Bashian, Jeremy Stern, Touqir Zahra, Faten Gad, Stephanie Chirico, Atosa Ahmadi, Alan J. Fischman, James E. Muller, and Michael R. Hamblin "Photosensitizer delivery to vulnerable atherosclerotic plaque: comparison of macrophage-targeted conjugate versus free chlorine(e6)," Journal of Biomedical Optics 11(2), 021008 (1 March 2006). https://doi.org/10.1117/1.2186039
Published: 1 March 2006
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 16 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Arteries
Luminescence
Tissues
Natural surfaces
Picosecond phenomena
Tissue optics
Molecules
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top