Computational fluorescence lifetime imaging microscopy

Liang Gao

doi:10.1117/12.3027279

4 October 2024 Computational fluorescence lifetime imaging microscopy

Liang Gao

Author Affiliations +

Proceedings Volume PC13118, Emerging Topics in Artificial Intelligence (ETAI) 2024; PC131180W (2024) https://doi.org/10.1117/12.3027279
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Abstract

Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence lifetimes of fluorescent probes to investigate molecular interactions. However, conventional FLIM systems often requires extensive scanning that is time-consuming. To address this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach acquires volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps. We demonstrated LIFT-FLIM using a single-photon avalanche diode array on various biological systems. Additionally, we expanded to spectral FLIM and demonstrated high-content multiplexed imaging of lung organoids. LIFT-FLIM can open new avenues in the biomedical research.

Conference Presentation

Citation Download Citation

Liang Gao "Computational fluorescence lifetime imaging microscopy", Proc. SPIE PC13118, Emerging Topics in Artificial Intelligence (ETAI) 2024, PC131180W (4 October 2024); https://doi.org/10.1117/12.3027279

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