Feeling inside living cells using active optical forces and passive interferometric particle tracking

Till M. Muenker; Gabriel Knotz; Lukas Abegg; Matthias Krüger; Timo Betz

doi:10.1117/12.3026761

3 October 2024 Feeling inside living cells using active optical forces and passive interferometric particle tracking

Till M. Muenker, Gabriel Knotz, Lukas Abegg, Matthias Krüger, Timo Betz

Proceedings Volume PC13112, Optical Trapping and Optical Micromanipulation XXI; PC131120W (2024) https://doi.org/10.1117/12.3026761
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Abstract

Living cells are among the most complex systems studied from both biological and physical perspectives. While cell biology has made tremendous advances in understanding the molecular composition of cells, our understanding of the physical characteristics within cells is not as advanced. We use optical tweezer-based active and passive microrheology to characterize the intracellular viscoelasticity and active forces, revealing significant variability in physical properties among different cell types. Furthermore, to facilitate passive microrheology in active systems, we introduce a novel stochastic observable based on a three-point correlations function. We will discuss these innovative approaches and the underlying analysis, which paves the way for a deeper physical understanding of living materials through "feeling" the cells using optical forces.

Conference Presentation

Citation Download Citation

Till M. Muenker, Gabriel Knotz, Lukas Abegg, Matthias Krüger, and Timo Betz "Feeling inside living cells using active optical forces and passive interferometric particle tracking", Proc. SPIE PC13112, Optical Trapping and Optical Micromanipulation XXI, PC131120W (3 October 2024); https://doi.org/10.1117/12.3026761

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