Optical tweezers for microrheology of the cumulus matrix in a microlitre volume

Chris Perrella; Carl A. Campugan; Tania Mendonca; Cheow Y. Tan; Megan Lim; Erik P. Schartner; Yoshihiko Arita; Graham D. Bruce; Amanda J. Wright; Kishan Dholakia; Kylie R. Dunning

doi:10.1117/12.3027394

3 October 2024 Optical tweezers for microrheology of the cumulus matrix in a microlitre volume

Chris Perrella, Carl A. Campugan, Tania Mendonca, Cheow Y. Tan, Megan Lim, Erik P. Schartner, Yoshihiko Arita, Graham D. Bruce, Amanda J. Wright, Kishan Dholakia, Kylie R. Dunning

Author Affiliations +

Proceedings Volume PC13112, Optical Trapping and Optical Micromanipulation XXI; PC131120V (2024) https://doi.org/10.1117/12.3027394
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Abstract

In the field of in vitro fertilisation (IVF), there is a need to understand how cell properties of the cumulus oocyte complex may be used to predict successful pregnancy and live birth rates post-IVF. Here we used optical tweezers for the first time to measure the viscosity of the cumulus cell matrix surrounding the oocyte (egg). This study aimed to determine whether the viscosity of the cumulus cell matrix – prior to fertilisation – is reflective of subsequent embryo developmental potential and indicative of pregnancy success. Measurements were performed using a 1µm diameter silica probe particle trapped by a focused 1064nm laser. We benchmarked the accuracy of the system by measuring the viscosity of glycerol with varying mass fractions. Viscosity measurements of the cumulus cell matrix were performed in isolation from both the cumulus cells and the oocyte. This showed that the viscosity of cumulus matrix was significantly higher when sampled from oocytes with a higher developmental potential (in vivo matured) compared to those of lower quality (in vitro matured).

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Chris Perrella, Carl A. Campugan, Tania Mendonca, Cheow Y. Tan, Megan Lim, Erik P. Schartner, Yoshihiko Arita, Graham D. Bruce, Amanda J. Wright, Kishan Dholakia, and Kylie R. Dunning "Optical tweezers for microrheology of the cumulus matrix in a microlitre volume", Proc. SPIE PC13112, Optical Trapping and Optical Micromanipulation XXI, PC131120V (3 October 2024); https://doi.org/10.1117/12.3027394

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KEYWORDS

Matrices

Viscosity

Optical tweezers

In vitro testing

Beam diameter

In vivo imaging

Particles

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