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3 October 2024 Magnetic micromanipulation of chromatin in live cells
Veer Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aizel, Maud Bongaerts, Lorena Kolar-Znika, Vittore Scolari, Edward J. Banigan, Leonid Mirny, Maxime Dahan, Daniele Fachinetti, Antoine Coulon, Daniel Larson
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Proceedings Volume PC13112, Optical Trapping and Optical Micromanipulation XXI; PC1311205 (2024) https://doi.org/10.1117/12.3029469
Event: Nanoscience + Engineering, 2024, San Diego, California, United States
Abstract
To gain a better understanding of material properties of chromatin and successfully link them to chromatin organization and functions such as transcription, we develop novel methods to actively manipulate a genomic locus inside the nucleus of a living human cell. By targeting iron-containing nanoparticles to a specific genomic locus and applying a controlled magnetic field, we were able to physically move chromatin through nuclear space for the first time. Exertion of near-picoNewton forces led to displacements over microns within minutes. We observe partially reversible stretching of chromatin highlighting its’ viscoelastic nature. We could accurately recapitulate the observed behavior with a Rouse model that included only a weak obstructive effect of the surrounding chromatin and nucleoplasmic material. This challenges the view that interphase chromatin is a gel-like material.
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Veer Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aizel, Maud Bongaerts, Lorena Kolar-Znika, Vittore Scolari, Edward J. Banigan, Leonid Mirny, Maxime Dahan, Daniele Fachinetti, Antoine Coulon, and Daniel Larson "Magnetic micromanipulation of chromatin in live cells", Proc. SPIE PC13112, Optical Trapping and Optical Micromanipulation XXI, PC1311205 (3 October 2024); https://doi.org/10.1117/12.3029469
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KEYWORDS
Magnetism
Genomics
Materials properties
Nanoparticles
Viscoelasticity
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