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3 October 2024 Automatic optical tweezers: advanced applications
Martin Selin, Antonio Ciarlo, Giuseppe Pesce, Marcel Rey, Lars Bengtsson, Joan Camuñas-Soler, Vinoth Sundar Rajan, Fredrik Westerlund, Marcus Wilhelmsson, Isabel Pastor, Felix Ritort, Steven B. Smith, Carlos Bustamante, Giovanni Volpe
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Proceedings Volume PC13112, Optical Trapping and Optical Micromanipulation XXI; PC1311203 (2024) https://doi.org/10.1117/12.3027752
Event: Nanoscience + Engineering, 2024, San Diego, California, United States
Abstract
Optical tweezers have become ubiquitous tools in science with use in disciplines ranging from biology to physics, chemistry, and material sciences with thousands of users around the world and a continuously growing number of applications. Here we show how a specially designed instrument, called miniTweezers2.0, can be made both highly versatile and user friendly. We demonstrate the system on three different experiments, which thanks to the close integration of the various parts of the tweezers into a single software are performed largely autonomously. The first experiment involves DNA stretching, a fundamental single molecule force spectroscopy experiment. The second experiment involved the stretching of red blood cells, which can be used to gauge the membrane stiffness of the cells. Lastly, we investigate the interaction between core-shell particles in various environments. Showing how the soft polymer layer extends, or contracts depending on pH and salinity. Our work show potential of automated and versatile optical tweezers systems in advancing our understanding of nano and micro-scale systems.
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© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Martin Selin, Antonio Ciarlo, Giuseppe Pesce, Marcel Rey, Lars Bengtsson, Joan Camuñas-Soler, Vinoth Sundar Rajan, Fredrik Westerlund, Marcus Wilhelmsson, Isabel Pastor, Felix Ritort, Steven B. Smith, Carlos Bustamante, and Giovanni Volpe "Automatic optical tweezers: advanced applications", Proc. SPIE PC13112, Optical Trapping and Optical Micromanipulation XXI, PC1311203 (3 October 2024); https://doi.org/10.1117/12.3027752
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KEYWORDS
Optical tweezers
Polymers
Equipment
Molecular spectroscopy
Molecules
Particles
Physics
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