STED microscopy at the tip of a holographic multimode fibre endoscope

André D. Gomes; Miroslav Stibůrek; Sergey Turtaev; Katharina Reglinski; Christian Eggeling; Tomáš Čižmár

doi:10.1117/12.3022278

20 June 2024 STED microscopy at the tip of a holographic multimode fibre endoscope

André D. Gomes, Miroslav Stibůrek, Sergey Turtaev, Katharina Reglinski, Christian Eggeling, Tomáš Čižmár

Proceedings Volume PC13007, Neurophotonics II; PC1300702 (2024) https://doi.org/10.1117/12.3022278
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Holographic multimode fibre endoscopes have recently shown their promising potential as a minimally invasive imaging tool, particularly in the field of neurobiology. Currently, their resolution is limited by diffraction, constrained by the relatively low numerical aperture of the multimode fibres used (typically less than 0.4). Overcoming the diffraction-limit barrier would open new possibilities for detailed observation of dendritic spines and their motility in-vivo. In this presentation we demonstrate pulsed STED microscopy delivered through a holographic multimode fibre endoscope. We show resolution improvements over 3-fold of the diffraction limit. Moreover, we discuss and showcase its applicability to bio-imaging.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

André D. Gomes, Miroslav Stibůrek, Sergey Turtaev, Katharina Reglinski, Christian Eggeling, and Tomáš Čižmár "STED microscopy at the tip of a holographic multimode fibre endoscope", Proc. SPIE PC13007, Neurophotonics II, PC1300702 (20 June 2024); https://doi.org/10.1117/12.3022278

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KEYWORDS

Multimode fibers

Endoscopes

Holography

Stimulated emission depletion microscopy

Microscopy

In vivo imaging

Brain

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