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18 June 2024 Joint estimation of calibration bead’s parameters and monochromatic aberrations in in-line digital holographic microscopy
Sachin Joshi, Dylan Brault, Thomas Olivier, Loïc Méès, Corinne Fournier
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Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC1299609 (2024) https://doi.org/10.1117/12.3016943
Event: SPIE Photonics Europe, 2024, Strasbourg, France
Abstract
In-line Digital holographic microscopy is easy to implement because it only requires a coherent illumination source. An out-of-focus image (hologram) of the sample is recorded and numerically reconstructed to retrieve phase shift information due to the sample. Effects of the optical setup, such as aberrations, can distort the hologram signal, and ignoring these effects results in biased reconstructions. In this context, we study the effects of misalignment and wrong cover-slip thickness by statistically analysing the reconstructions of beads distributed in the whole field of view. Our aberration-wise reconstruction approach produces debiased estimations and allows precise and realistic estimation of optical aberrations from a single hologram.
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Sachin Joshi, Dylan Brault, Thomas Olivier, Loïc Méès, and Corinne Fournier "Joint estimation of calibration bead’s parameters and monochromatic aberrations in in-line digital holographic microscopy", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC1299609 (18 June 2024); https://doi.org/10.1117/12.3016943
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KEYWORDS
Digital holography
Optical aberrations
Holography
Microscopy
Monochromatic aberrations
Calibration
Holograms
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