A novel coupling of MALDI imaging and fast QCL-based IR imaging for disease detection

Thomas J. Tague Jr.; Peng Wang; Niels Kroeger-Lui; Ethan Yang; Joshua L. Fischer

doi:10.1117/12.3004823

13 March 2024 A novel coupling of MALDI imaging and fast QCL-based IR imaging for disease detection

Thomas J. Tague Jr., Peng Wang, Niels Kroeger-Lui, Ethan Yang, Joshua L. Fischer

Proceedings Volume PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; PC128620N (2024) https://doi.org/10.1117/12.3004823
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Molecular analysis of tissue samples plays an important role in understanding basic functionality of organisms and detecting abnormality. Acquiring comprehensive information on heterogeneous and complex biological systems with unique chemical specificity and high spatial resolution provides a powerful tool for early-stage disease detection and monitoring. However, MALDI mass spectrometry imaging of whole tissue sections at high spatial resolution (≤10 μm pixel size) requires extensive acquisition time. Here, Bruker’s proposed end-to-end workflow for quantum cascade laser (QCL) based infrared (IR) guided MALDI imaging was employed to obtain deep molecular information on various tissue samples. MALDI imaging is more sensitive and provides deeper molecular information than IR based imaging, but QCL-IR based imaging is much faster. QCL-IR imaging is therefore used as a screening tool for disease detection, where if disease is detected, then the more time-consuming MALDI imaging can target only the region of the tissue section in question. Examples presented include a proof-of-concept mouse brain sample and a practical multistage pancreatic cancer tissue sample. This approach facilitates region of interest (ROI) selection and significantly increases sample throughput – thereby enabling large-scale MALDI imaging studies in cancer research.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Thomas J. Tague Jr., Peng Wang, Niels Kroeger-Lui, Ethan Yang, and Joshua L. Fischer "A novel coupling of MALDI imaging and fast QCL-based IR imaging for disease detection", Proc. SPIE PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, PC128620N (13 March 2024); https://doi.org/10.1117/12.3004823

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KEYWORDS

Infrared imaging

Chemical analysis

Diseases and disorders

Tissues

Imaging spectroscopy

Quantum cascade lasers

Quantum experiments

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