Using water as immersion medium for scanning laser optical tomography to analyze functionalized silicone waveguides

Bjoern Ole Hill; Tobias Biermann; Tammo Ripken; Roland Lachmayer; Merve Wollweber

doi:10.1117/12.3003809

13 March 2024 Using water as immersion medium for scanning laser optical tomography to analyze functionalized silicone waveguides

Bjoern Ole Hill, Tobias Biermann, Tammo Ripken, Roland Lachmayer, Merve Wollweber

Author Affiliations +

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC128480D (2024) https://doi.org/10.1117/12.3003809
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Refractive index matching is typically achieved with combinations of silicone oils in Scanning Laser Optical Tomography. This leads to destruction of some types of samples such as silicone waveguides. A correction method was developed which allows image acquisition with high quality without perfectly matching the refractive index of medium and sample. This enables the use of water as the immersion medium instead of silicone oil. Promising results with high image quality are presented.

Conference Presentation

Citation Download Citation

Bjoern Ole Hill, Tobias Biermann, Tammo Ripken, Roland Lachmayer, and Merve Wollweber "Using water as immersion medium for scanning laser optical tomography to analyze functionalized silicone waveguides", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC128480D (13 March 2024); https://doi.org/10.1117/12.3003809

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