CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC12847 > Article
Presentation
13 March 2024 Supercontinuum intrinsic fluorescence imaging (SCIFI): bringing multiphoton microscopy closer to commercialization?
Haohua Tu
Author Affiliations +
Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470B (2024) https://doi.org/10.1117/12.3002974
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Despite the long development of light microscopy, it remains challenging to visualize fragile living systems without labeling or specific sample preparation. Taking hint from the supercontinuum generation in ultrafast laser engineering, we generated ‘supercontinuum’ fluorescence from unlabeled live samples before nonlinear phototoxicity onset. Our supercontinuum intrinsic fluorescence imaging (SCIFI) identified drug cytotoxicity in real-time, demystified embryogenesis before/after implantation, performed omics-like cell phenotyping or tissue histopathology, tracked freely moving small organisms, scanned brain area for targeted patching, and provided new insights on photosynthesis. This demonstration paves the way for a mobile microscopy facility to freely exploit molecular biology across life sciences.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Haohua Tu "Supercontinuum intrinsic fluorescence imaging (SCIFI): bringing multiphoton microscopy closer to commercialization?", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470B (13 March 2024); https://doi.org/10.1117/12.3002974
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Fluorescence imaging
Multiphoton microscopy
Commercialization
Femtosecond pulse shaping
Molecular imaging
Multiphoton fluorescence microscopy
Pulse signals
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top