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17 March 2023 Quantitation of an expanded SERS nanoparticle library for highly multiplexed imaging assays (Conference Presentation)
Alexander Czaja, Samer Awad, Olga Eremina, Cristina L. Zavaleta
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Proceedings Volume PC12395, Colloidal Nanoparticles for Biomedical Applications XVIII; PC1239509 (2023) https://doi.org/10.1117/12.2648091
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
SERS nanoparticles are powerful optical contrast agents for imaging assays. Their highly specific sets of narrow spectral bands make them well suited for multiplexing applications, and their enhanced inelastic scattering cross sections enable rapid, high content imaging. Multiplexed hyperspectral imaging datasets commonly undergo a spectral unmixing postprocessing step using a compensation matrix of reference spectra to produce quantitative image channels. We perform hyperspectral Raman imaging on mixtures with increasing plexity and varying degrees of linear system conditioning and compare against the ground truth to determine the most robust workflow for quantitative biological SERS imaging.
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Alexander Czaja, Samer Awad, Olga Eremina, and Cristina L. Zavaleta "Quantitation of an expanded SERS nanoparticle library for highly multiplexed imaging assays (Conference Presentation)", Proc. SPIE PC12395, Colloidal Nanoparticles for Biomedical Applications XVIII, PC1239509 (17 March 2023); https://doi.org/10.1117/12.2648091
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KEYWORDS
Multiplexing
Nanoparticles
Imaging systems
Hyperspectral imaging
Raman scattering
Gold
Raman spectroscopy
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