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Raman spectroscopy provides a comprehensive molecular information of a biological sample without any invasive and destructive treatment prior to a measurement. However, the slow measurement speed limits imaging applications of Raman spectroscopy. I present acceleration of Raman spectroscopy and imaging by multiplexed spatial sampling. Multiline illumination Raman microscopy, scanning a sample with multiple line-shaped laser foci, improved the imaging speed by 4 orders of magnitude in comparison to conventional confocal Raman microscopy. I also present a selective-sampling approach for accelerating Raman spectroscopy and imaging. I discuss the potential new applications of accelerated Raman spectroscopy and imaging in life sciences.
Yasuaki Kumamoto
"Accelerating spontaneous Raman spectroscopy and imaging (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920I (15 March 2023); https://doi.org/10.1117/12.2650181
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Yasuaki Kumamoto, "Accelerating spontaneous Raman spectroscopy and imaging (Conference Presentation)," Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920I (15 March 2023); https://doi.org/10.1117/12.2650181