A UV-C LED-based reactor for continuous decontamination of the sheath fluid in a flow-cytometric cell sorter (Conference Presentation)

Konrad von Volkmann; Jenny Kirsch; Kerstin Heinrich; Benjamin Grothe; Johannes Glaab; Toralf Kaiser

doi:10.1117/12.2650313

15 March 2023 A UV-C LED-based reactor for continuous decontamination of the sheath fluid in a flow-cytometric cell sorter (Conference Presentation)

Konrad von Volkmann, Jenny Kirsch, Kerstin Heinrich, Benjamin Grothe, Johannes Glaab, Toralf Kaiser

Author Affiliations +

Proceedings Volume PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; PC1238305 (2023) https://doi.org/10.1117/12.2650313
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Aseptic cell sorting is important for cell culture and downstream analyses of sorted cells. However, keeping the sort aseptic is challenging as the sheath fluid in a cell sorter may easily be contaminated with germs such as bacteria, yeasts, viruses, or fungi. Thus, a regular chemical cleaning of the whole fluidics system is required. However, this procedure is time consuming, and its success can hardly be verified. Here, we present a method for sheath fluid decontaminated by irradiation with UV-C light using a flow-through principle. With this principle, a 5 log10 reduction of bacteria in the sheath fluid was achieved.

Conference Presentation

Citation Download Citation

Konrad von Volkmann, Jenny Kirsch, Kerstin Heinrich, Benjamin Grothe, Johannes Glaab, and Toralf Kaiser "A UV-C LED-based reactor for continuous decontamination of the sheath fluid in a flow-cytometric cell sorter (Conference Presentation)", Proc. SPIE PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, PC1238305 (15 March 2023); https://doi.org/10.1117/12.2650313

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