Recently developed mid-infrared photothermal (MIP) microscopy (for a Review, Science Advances, 2021, 7: eabg1559) not only overcomes the diffraction limit in direct IR imaging, but also circumvents the limitations in AFM-IR. In MIP microscopy, a visible beam probes the thermal effects induced by an intensity-modulated infrared beam. The MIP signals are measured in scanner manner or in wide-field manner. Video rate MIP imaging of living cells and tissues has been reached through a single pulse digitization approach. In this presentation, I will discuss the principle, instrumentation, and applications of MIP microscopy.
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