Volumetric longitudinal live imaging with a multi-modal platform to monitor the morphological and functional development of human heart organoids

Kibeom Park; Senyue Hao; Fei Wang; Volmert Brett; Marcello Amaral; Yilin Li; Aguirre Aitor; Chao Zhou

doi:10.1117/12.2652957

10 March 2023 Volumetric longitudinal live imaging with a multi-modal platform to monitor the morphological and functional development of human heart organoids

Kibeom Park, Senyue Hao, Fei Wang, Volmert Brett, Marcello Amaral, Yilin Li, Aguirre Aitor, Chao Zhou

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Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671F (2023) https://doi.org/10.1117/12.2652957
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Longitudinal imaging of live organisms is essential to life science research aiming to understand biological processes. Recently, the imaging system has been housed for longitudinal imaging by mounting the optical imaging system in an incubator, while facing the limitations of single-modality and low throughput. Here, we proposed the OCToScope, a multi-modal, high-throughput and compact platform that is compatible with commercial incubators. OCToScope incorporates Optical Coherence Tomography(OCT) to achieve non-invasive three-dimensional imaging, and fluorescent imaging that retrieves cell-specific information. OCToScope also supports automatic scanning of mass samples quantity with motorized x-y-z stage and perform high time-resolution imaging for long-term studies. we used OCToScope to provide a systematic and comprehensive characterization of human heart organoids for over 30 days without any interference.

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Kibeom Park, Senyue Hao, Fei Wang, Volmert Brett, Marcello Amaral, Yilin Li, Aguirre Aitor, and Chao Zhou "Volumetric longitudinal live imaging with a multi-modal platform to monitor the morphological and functional development of human heart organoids", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671F (10 March 2023); https://doi.org/10.1117/12.2652957

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KEYWORDS

Heart

Multimodal imaging

Imaging systems

Light emitting diodes

Light sources

Optical filters

Optical imaging

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