Albert Claude Boccara,1 Olivier Thouvenin,1 Amir H. Gandjbakhche,2 Linh Giang PHAM,3 Martine Boccara4
1Institut Langevin Ondes et Images (France) 2National Institutes of Health (United States) 3Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France) 4Institut de biologie de l'Ecole Normale Supérieure (France)
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Whereas Full Field OCT (FFOCT) relies on backscattering of light, Full Field Optical Transmission Tomography (FFOTT) relies on forward scattering using the Gouy’s phase shift modulation that is achieved close to the focus of a microscope objective. This new type of endogenous cell imaging technique that offers structural and metabolic contrasts is particularly well suited for imaging cell culture on glass slide or Petri dishes avoiding fringes that mask cells in FFOCT as well as biological structures such as biofilms. The sectioning ability is close to confocal microscopy but no contrast agent is required.
Albert Claude Boccara,Olivier Thouvenin,Amir H. Gandjbakhche,Linh Giang PHAM, andMartine Boccara
"Full field optical transmission tomography (FFOTT) of biological samples: Static and dynamic approaches", Proc. SPIE PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, PC119480R (7 March 2022); https://doi.org/10.1117/12.2607852
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Albert Claude Boccara, Olivier Thouvenin, Amir H. Gandjbakhche, Linh Giang PHAM, Martine Boccara, "Full field optical transmission tomography (FFOTT) of biological samples: Static and dynamic approaches," Proc. SPIE PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, PC119480R (7 March 2022); https://doi.org/10.1117/12.2607852