GPU-based rapid reconstruction of cellular 3D refractive index maps from tomographic phase microscopy
(Conference Presentation)

Gili Dardikman; Natan T. Shaked

doi:10.1117/12.2209315

27 April 2016 GPU-based rapid reconstruction of cellular 3D refractive index maps from tomographic phase microscopy (Conference Presentation)

Gili Dardikman, Natan T. Shaked

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180G (2016) https://doi.org/10.1117/12.2209315
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present highly parallel and efficient algorithms for real-time reconstruction of the quantitative three-dimensional (3-D) refractive-index maps of biological cells without labeling, as obtained from the interferometric projections acquired by tomographic phase microscopy (TPM). The new algorithms are implemented on the graphic processing unit (GPU) of the computer using CUDA programming environment. The reconstruction process includes two main parts. First, we used parallel complex wave-front reconstruction of the TPM-based interferometric projections acquired at various angles. The complex wave front reconstructions are done on the GPU in parallel, while minimizing the calculation time of the Fourier transforms and phase unwrapping needed. Next, we implemented on the GPU in parallel the 3-D refractive index map retrieval using the TPM filtered-back projection algorithm. The incorporation of algorithms that are inherently parallel with a programming environment such as Nvidia’s CUDA makes it possible to obtain real-time processing rate, and enables high-throughput platform for label-free, 3-D cell visualization and diagnosis.

Conference Presentation

Citation Download Citation

Gili Dardikman and Natan T. Shaked "GPU-based rapid reconstruction of cellular 3D refractive index maps from tomographic phase microscopy (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180G (27 April 2016); https://doi.org/10.1117/12.2209315

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KEYWORDS

Reconstruction algorithms

Computer programming

Microscopy

Refractive index

Tomography

Interferometry

3D visualizations

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