CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 9714 > Article
Presentation
27 April 2016 Study conformational dynamics of intrinsically disordered protein by PET-FCS (Conference Presentation)
Joerg Enderlein, Man Zhou, Qui Van, Ingo Gregor
Author Affiliations +
Proceedings Volume 9714, Single Molecule Spectroscopy and Superresolution Imaging IX; 971404 (2016) https://doi.org/10.1117/12.2212247
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
Intrinsically disordered proteins (IDP) form a large and functionally important class of proteins that lack an ordered three-dimensional structure. IDPs play an important role in cell signaling, transcription, or chromatin remodeling. The discovery of IDPs has challenged the traditional paradigm of protein structure which states that protein function depends on a well-defined three-dimensional structure. Due to their high conformational flexibility and the lack of ordered secondary structure, it is challenging to study the flexible structure, dynamics and energetics of these proteins with conventional methods. In our work, we employ photoinduced electron transfer (PET) combined with fluorescence correlation spectroscopy (FCS) for studying the conformational dynamics of one specific class of IDPs: phenylalanine-glycine rich protein domains (FG repeats) which are dominant building blocks within the pore of nuclear pore complexes. Nuclear pore complexes are large protein assemblies that cross the nuclear envelope and form selective barrier, which regulate bidirectional exchange between nucleus and cytoplasm.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Joerg Enderlein, Man Zhou, Qui Van, and Ingo Gregor "Study conformational dynamics of intrinsically disordered protein by PET-FCS (Conference Presentation)", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 971404 (27 April 2016); https://doi.org/10.1117/12.2212247
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Proteins
Fluorescence correlation spectroscopy
Imaging spectroscopy
Positron emission tomography
Current controlled current source
Single molecule spectroscopy
Super resolution
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top