Excimer Laser Modification Of Plastic Surfaces For Biotechnological Applications

A. Akerman; G. Keen; D. I. Klick; J. A. O'Neill; G. L. Paul; G. Wilson; R. Gregson

doi:10.1117/12.947706

24 October 1988 Excimer Laser Modification Of Plastic Surfaces For Biotechnological Applications

A. Akerman, G. Keen, D. I. Klick, J. A. O'Neill, G. L. Paul, G. Wilson, R. Gregson

Proceedings Volume 0957, Laser Beam Surface Treating and Coating; (1988) https://doi.org/10.1117/12.947706
Event: SPIE International Symposium on Optical Engineering and Industrial Sensing for Advance Manufacturing Technologies, 1988, Dearborn, MI, United States

Abstract

Binding of proteins to polystyrene plates used in enzyme linked immunosorbent assays (Elisa tests) has been improved by three different methods using UV radiation from an excimer laser. The methods studied were: i) a high fluence method, where material was ablated from the surface using focussed radiation, ii) a low fluence method, where de-focussed radiation was used to form new chemical species photochemically without material removal, and iii) a coating process, where the UV radiation was used to cure a surface coating on the plates which contained functional groups which would provide good sites for bonding. All three processes have been studied over a broad range of experimental parameters, and conditions leading to improved binding of protein have been determined for each process.

Citation Download Citation

A. Akerman, G. Keen, D. I. Klick, J. A. O'Neill, G. L. Paul, G. Wilson, and R. Gregson "Excimer Laser Modification Of Plastic Surfaces For Biotechnological Applications", Proc. SPIE 0957, Laser Beam Surface Treating and Coating, (24 October 1988); https://doi.org/10.1117/12.947706

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