Rapid detection of EBOLA VP40 in microchip immunofiltration assay

Peter Miethe; Dominik Gary; Nadine Hlawatsch; Anne-Marie Gad

doi:10.1117/12.2184649

21 May 2015 Rapid detection of EBOLA VP40 in microchip immunofiltration assay

Peter Miethe, Dominik Gary, Nadine Hlawatsch, Anne-Marie Gad

Proceedings Volume 9490, Advances in Global Health through Sensing Technologies 2015; 94900H (2015) https://doi.org/10.1117/12.2184649
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

In the spring of 2014, the Ebola virus (EBOV) strain Zaire caused a dramatic outbreak in several regions of West Africa. The RT-PCR and antigen capture diagnostic proved to be effective for detecting EBOV in blood and serum. In this paper, we present data of a rapid antigen capture test for the detection of VP40. The test was performed in a microfluidic chip for immunofiltration analysis. The chip integrates all necessary assay components. The analytical sensitivity of the rapid test was 8 ng/ml for recombinant VP40. In serum and whole blood samples spiked with virus culture material, the detection limit was 2.2 x 10² PFU/ml. The performance data of the rapid test (15 min) are comparable to that of the VP40 laboratory ELISA.

Citation Download Citation

Peter Miethe, Dominik Gary, Nadine Hlawatsch, and Anne-Marie Gad "Rapid detection of EBOLA VP40 in microchip immunofiltration assay", Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900H (21 May 2015); https://doi.org/10.1117/12.2184649

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