Quantitative phase recovery from asymmetric illumination on an LED array microscope

Lei Tian; Laura Waller

doi:10.1117/12.2078286

11 March 2015 Quantitative phase recovery from asymmetric illumination on an LED array microscope

Lei Tian, Laura Waller

Author Affiliations +

Proceedings Volume 9336, Quantitative Phase Imaging; 93360A (2015) https://doi.org/10.1117/12.2078286
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Differential phase contrast (DPC) is a quantitative phase imaging technique which measures the sample’s phase derivative by taking two images from complementary asymmetric illumination patterns. Distinct from coherent techniques, DPC relies on partially coherent illumination, providing 2× better lateral resolution, better optical sectioning, and immunity to speckle noise. In this paper, we derive the weak object transfer function to quantify how sample’s phase is converted into our DPC measurements, then develop quantitative inversion methods. Phase reconstructions from single-axis DPC measurements suffer from missing frequencies along the axis of asymmetry. We measure the missing frequency information by taking DPC measurements from other axes. Our phase reconstruction method provides a unified framework for both single and multi-axis DPC measurements. We implement our DPC measurements in real-time and along arbitrary axes of asymmetry by computational illumination on an LED array microscope.

Citation Download Citation

Lei Tian and Laura Waller "Quantitative phase recovery from asymmetric illumination on an LED array microscope", Proc. SPIE 9336, Quantitative Phase Imaging, 93360A (11 March 2015); https://doi.org/10.1117/12.2078286

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE