A novel antibody immobilization strategy for optical biosensors

Mark A. Lifson; Jared A. Carter; Benjamin L. Miller

doi:10.1117/12.2083877

2 March 2015 A novel antibody immobilization strategy for optical biosensors

Mark A. Lifson, Jared A. Carter, Benjamin L. Miller

Proceedings Volume 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII; 931007 (2015) https://doi.org/10.1117/12.2083877
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Arrayed Imaging Reflectometry (AIR) is a highly sensitive label-free biosensor which can be used to detect hundreds of antigens on a single substrate. The signal monitored with AIR is the light intensity of an angled beam reflected off of a flat substrate which is composed of a protein-reactive film on a thermally grown silicon oxide layer. If the angle, wavelength, and polarization of the incident light beam is fixed, a near-zero reflectance condition can be obtained by adjusting the thickness of the thermally grown oxide. In a typical AIR biosensing experiment, antibodies are printed (using a piezoelectric microarrayer) on top of the oxide layer to create a minimum reflectance condition. If the substrate is exposed to a complex solution (such as serum), the patterned antibodies bind to their specific targets increasing the effective spot thickness, which perturbs the anti-reflective condition and causes a measurable signal increase. One of the main considerations with AIR is evaluating and controlling the bioactivity and efficiency of antibody immobilization after printing, since these factors significantly affect the dynamic range and limit of detection. Here, we present preliminary experiments towards using microgel nanoparticles as a simple and customizable construct to deposit antibodies on biosensor surfaces. This method can be generalized to work with other microarray technology formats, including those that are not label-free.

Citation Download Citation

Mark A. Lifson, Jared A. Carter, and Benjamin L. Miller "A novel antibody immobilization strategy for optical biosensors", Proc. SPIE 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII, 931007 (2 March 2015); https://doi.org/10.1117/12.2083877

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