A novel transmission electron imaging technique for observation of biological samples on a plate

Yusuke Ominami; Masato Nakajima M.D.; Tatsuo Ushiki M.D.; Sukehiro Ito

doi:10.1117/12.2065479

16 September 2014 A novel transmission electron imaging technique for observation of biological samples on a plate

Yusuke Ominami, Masato Nakajima M.D., Tatsuo Ushiki M.D., Sukehiro Ito

Proceedings Volume 9236, Scanning Microscopies 2014; 923608 (2014) https://doi.org/10.1117/12.2065479
Event: SPIE Scanning Microscopies, 2014, Monterey, California, United States

Abstract

We introduce a novel transmission electron imaging method to clarify the internal structure of single whole cells by scanning electron microscopy (SEM). In this method, whole cells are cultivated on a transparent flat plate in advance, which is made of a scintillator that emits photons by irradiating an electron beam. The detector developed here can obtain both the secondary electron (SE) surface images and transmission electron (TE) images. Observations of whole mount cells by this technique clearly show that the cellular internal structure can be observed as transmission images which are produced by photons emitted from the scintillator plate by electron beam irradiation.

Citation Download Citation

Yusuke Ominami, Masato Nakajima M.D., Tatsuo Ushiki M.D., and Sukehiro Ito "A novel transmission electron imaging technique for observation of biological samples on a plate", Proc. SPIE 9236, Scanning Microscopies 2014, 923608 (16 September 2014); https://doi.org/10.1117/12.2065479

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