Some unique features of alkaliphilic anaerobes

Erin Roof; Elena Pikuta; Christopher Otto; George Williams; Richard Hoover

doi:10.1117/12.2045350

26 September 2013 Some unique features of alkaliphilic anaerobes

Erin Roof, Elena Pikuta, Christopher Otto, George Williams, Richard Hoover

Proceedings Volume 8865, Instruments, Methods, and Missions for Astrobiology XVI; 88650F (2013) https://doi.org/10.1117/12.2045350
Event: SPIE Optical Engineering + Applications, 2013, San Diego, California, United States

Abstract

This article explores two topics involving the examination of four strains of alkaliphilic anaerobes. The first topic was dedicated to detection of the ability of microorganisms to metabolize alternative chirality substrates. Two saccharolytic anaerobic bacteria were chosen for the first experiment: Anaerovirgula multivorans strain SCAT, which is gram positive and spore-forming; and Spirochaeta dissipatitropha, strain ASpC2^T, which is gram negative. It was found that both checked sugarlytics were able to use L-ribose and L-arabinose, as growth substrates. The second part was concerned of study a chemolithotrophy in two halo-alkaliphilic sulfate reducing bacteria: Desulfonatornum thiodismutans strain MLF1^T and Desulfonatronum lacustre strain Z-7951^T. The experiments with lithotrophs had demonstrated that strain MLF1^Twas capable to grow without any organic source of carbon, while strain Z-7951^T had required at least 2 mM sodium acetate for growth. Anaerobic technique was used for preparation of the growth media and maintenance of these bacterial cultures. Standard methods for Gram, spore, and flagella staining were applied for characterization of cytomorphology. In this article, the results of the experiments performed on cytological, physiological, and biochemical levels are presented and discussed.

Citation Download Citation

Erin Roof, Elena Pikuta, Christopher Otto, George Williams, and Richard Hoover "Some unique features of alkaliphilic anaerobes", Proc. SPIE 8865, Instruments, Methods, and Missions for Astrobiology XVI, 88650F (26 September 2013); https://doi.org/10.1117/12.2045350

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