Raman spectroscopy for intracellular localisation of meso-tetraphenylporphyrin-gold nanoparticles conjugates

Rasoul Al-Majmaie; Nebras Alattar; Eamonn Kennedy; Mohamed Al-Rubeai; James H. Rice; Dominic Zerulla

doi:10.1117/12.2024240

20 September 2013 Raman spectroscopy for intracellular localisation of meso-tetraphenylporphyrin-gold nanoparticles conjugates

Rasoul Al-Majmaie, Nebras Alattar, Eamonn Kennedy, Mohamed Al-Rubeai, James H. Rice, Dominic Zerulla

Author Affiliations +

Proceedings Volume 8815, Nanoimaging and Nanospectroscopy; 88150K (2013) https://doi.org/10.1117/12.2024240
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

This study reports on the use of surface enhanced Raman scattering (SERS) as a non-destructive tool for detection and localisation of Porphyrin-Gold nanoparticles (GNP) conjugates at the subcellular level. Conjugates of the hydrophobic photosensitizer meso-Tetraphenylporphyrin (TPP) and GNPs were synthesized. The TPP-GNPs were characterized by by ultraviolet—visible absorption spectroscopy, fluorescence spectroscopy and transmission electron microscopy. TPPGNPs with a mean diameter of 12 nm were introduced into SW480 human colon adenocarcinoma cells. Single point SERS was applied in conjunction with fluorescence microscopy to localize the exogenous materials within the cells. Our results indicate that the TPP-GNP nanomaterials are distributed within cells in the cytoplasm. Overall our results indicate that Raman spectroscopy has the potential to be a high-throughput tool to localise nanoparticles in the subcellular environment.

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Rasoul Al-Majmaie, Nebras Alattar, Eamonn Kennedy, Mohamed Al-Rubeai, James H. Rice, and Dominic Zerulla "Raman spectroscopy for intracellular localisation of meso-tetraphenylporphyrin-gold nanoparticles conjugates", Proc. SPIE 8815, Nanoimaging and Nanospectroscopy, 88150K (20 September 2013); https://doi.org/10.1117/12.2024240

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KEYWORDS

Raman spectroscopy

Nanoparticles

Surface enhanced Raman spectroscopy

Luminescence

Transmission electron microscopy

Gold

Signal to noise ratio

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