Multispot two-photon imaging of calcium waves dynamics in  cardial tissue at 16Hz frame rate

C. de Mauro; C. A. Cecchetti; D. Alfieri; G. Borile; A. Urbani; M. Mongillo; F. S. Pavone

doi:10.1117/12.2032499

17 June 2013 Multispot two-photon imaging of calcium waves dynamics in cardial tissue at 16Hz frame rate

C. de Mauro, C. A. Cecchetti, D. Alfieri, G. Borile, A. Urbani, M. Mongillo, F. S. Pavone

Proceedings Volume 8797, Advanced Microscopy Techniques III; 87970L (2013) https://doi.org/10.1117/12.2032499
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dynamics at intracellular level. Time resolution of a few milliseconds, even in full frame images at 512×512 pixels, is achieved, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue.

Citation Download Citation

C. de Mauro, C. A. Cecchetti, D. Alfieri, G. Borile, A. Urbani, M. Mongillo, and F. S. Pavone "Multispot two-photon imaging of calcium waves dynamics in cardial tissue at 16Hz frame rate", Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970L (17 June 2013); https://doi.org/10.1117/12.2032499

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