Developing a cell-based sensor for the detection of Autoinducer-2

Matthew D. Servinsky; Katherine Germane; Elliot S. Gerlach; Chen-Yu Tsao; Christopher M. Byrd; Christian J. Sund; William E. Bentley

doi:10.1117/12.2016601

5 June 2013 Developing a cell-based sensor for the detection of Autoinducer-2

Matthew D. Servinsky, Katherine Germane, Elliot S. Gerlach, Chen-Yu Tsao, Christopher M. Byrd, Christian J. Sund, William E. Bentley

Author Affiliations +

Proceedings Volume 8719, Smart Biomedical and Physiological Sensor Technology X; 871903 (2013) https://doi.org/10.1117/12.2016601
Event: SPIE Defense, Security, and Sensing, 2013, Baltimore, Maryland, United States

Abstract

Bacteria use an intricate set of communication systems for sensing and interpreting environmental cues that coordinate population-based behavior. Quorum sensing is one of these systems, and it involves the production, release, and detection of small chemical signaling molecules. Recent research has revealed the role of quorum sensing molecules in the control of microbial activities such as biofilm formation. In this presentation we outline the development of a recombinant E. coli cell-based sensor for detection of the quorum sensing molecule Autoinducer-2 (AI-2), as well as engineering strategies to remove sugar and anoxic inhibition of the strain.

Citation Download Citation

Matthew D. Servinsky, Katherine Germane, Elliot S. Gerlach, Chen-Yu Tsao, Christopher M. Byrd, Christian J. Sund, and William E. Bentley "Developing a cell-based sensor for the detection of Autoinducer-2", Proc. SPIE 8719, Smart Biomedical and Physiological Sensor Technology X, 871903 (5 June 2013); https://doi.org/10.1117/12.2016601

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available