Molecular thermometers for potential applications in thermal ablation procedures

Natalia G. Zhegalova; Alex Aydt; Steven T. Wang; Mikhail Y. Berezin

doi:10.1117/12.2008784

21 February 2013 Molecular thermometers for potential applications in thermal ablation procedures

Natalia G. Zhegalova, Alex Aydt, Steven T. Wang, Mikhail Y. Berezin

Proceedings Volume 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V; 85960I (2013) https://doi.org/10.1117/12.2008784
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Thermal ablation is a promising minimally invasive method for treating tumors without surgical intervention. Thermal ablation uses thermal sources such as lasers, radiowaves or focused ultrasound to increase the temperature of the tumor to levels lethal to cancer cells. This treatment based on heat therapy may be problematic as the temperature of the operation site is unknown. To address this problem, we developed optical molecular thermometers that can potentially measure the temperature on a molecular scale and be compatible with in vivo measurements. The thermometers are centered on a combination of two fluorophores emitting in two distinct spectral ranges and having different temperature-dependent emission properties. In this design, a fluorophore with relatively insensitive temperature-dependent fluorescence serves as a reference while another sensitive fluorophore serves as a sensor. We have demonstrated the feasibility of this approach using a coumarin-rhodamine conjugate. The sensitivity of the construct to the clinically relevant ablation temperatures (20-85 °C) was demonstrated in vitro.

Citation Download Citation

Natalia G. Zhegalova, Alex Aydt, Steven T. Wang, and Mikhail Y. Berezin "Molecular thermometers for potential applications in thermal ablation procedures", Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 85960I (21 February 2013); https://doi.org/10.1117/12.2008784

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available