Whispering gallery mode aptasensors for detection of blood proteins

L. Pasquardini; S. Berneschi; A. Barucci; F. Cosi; M. Insinna; L. Lunelli; G. Nunzi Conti; C. Pederzolli; S. Salvadori; S. Soria

doi:10.1117/12.2001987

20 March 2013 Whispering gallery mode aptasensors for detection of blood proteins

L. Pasquardini, S. Berneschi, A. Barucci, F. Cosi, M. Insinna, L. Lunelli, G. Nunzi Conti, C. Pederzolli, S. Salvadori, S. Soria

Author Affiliations +

Proceedings Volume 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII; 85760U (2013) https://doi.org/10.1117/12.2001987
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was numerically modelled and optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.

Citation Download Citation

L. Pasquardini, S. Berneschi, A. Barucci, F. Cosi, M. Insinna, L. Lunelli, G. Nunzi Conti, C. Pederzolli, S. Salvadori, and S. Soria "Whispering gallery mode aptasensors for detection of blood proteins", Proc. SPIE 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII, 85760U (20 March 2013); https://doi.org/10.1117/12.2001987

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