Partial Inhibition Of Glycolysis With 2-Deoxy-D-Glucose Enhances EDKC Mediated Cellular Photosensitization.

G. Ara; D. Ohuoha; A. R. Oseroff

doi:10.1117/12.942712

19 February 1988 Partial Inhibition Of Glycolysis With 2-Deoxy-D-Glucose Enhances EDKC Mediated Cellular Photosensitization.

G. Ara, D. Ohuoha, A. R. Oseroff

Author Affiliations +

Proceedings Volume 0847, New Directions in Photodynamic Therapy; (1988) https://doi.org/10.1117/12.942712
Event: Cambridge Symposium on Optics in Medicine and Visual Image Processing, 1987, San Diego, CA, United States

Abstract

The effects of depleting glytolytic ATP on EDKC mediated cellular phototoxicity has been investigated using squamous carcinoma cells (FaDu) in vitro. Cells were pretreated with a non-metabolizable glucose analog 2-deoxy-D-glucose (5 mM) for 13-15 hr that resulted in 40-45 % decrease of cellular ATP. Subsequent exposure of the cells to EDKC resulted in twice as much dye uptake compared to control cells and upon radiation induced 4-5 fold more killing. Pretreatment with 2-deoxy-D-glucose (2-DG) alone did not have any effect on cell survival. We conclude that if cellular glycolytic ATP is lowered prior to their exposure to EDKC and radiation, cells become more vulnerable to the dye mediated photosensitization.

Citation Download Citation

G. Ara, D. Ohuoha, and A. R. Oseroff "Partial Inhibition Of Glycolysis With 2-Deoxy-D-Glucose Enhances EDKC Mediated Cellular Photosensitization.", Proc. SPIE 0847, New Directions in Photodynamic Therapy, (19 February 1988); https://doi.org/10.1117/12.942712

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