Raman microspectroscopy of algal lipid bodies: &#946;-carotene as a volume sensor

Zdenek Pilát; Silvie Bernatová; Jan Ježek; Mojmir Šery; Ota Samek; Pavel Zemánek; Ladislav Nedbal; Martin Trtílek

doi:10.1117/12.912264

11 October 2011 Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor

Zdenek Pilát, Silvie Bernatová, Jan Ježek, Mojmir Šery, Ota Samek, Pavel Zemánek, Ladislav Nedbal, Martin Trtílek

Proceedings Volume 8306, Photonics, Devices, and Systems V; 83060L (2011) https://doi.org/10.1117/12.912264
Event: Photonics Prague 2011, 2011, Prague, Czech Republic

Abstract

Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain β-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of β-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations.

Citation Download Citation

Zdenek Pilát, Silvie Bernatová, Jan Ježek, Mojmir Šery, Ota Samek, Pavel Zemánek, Ladislav Nedbal, and Martin Trtílek "Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor", Proc. SPIE 8306, Photonics, Devices, and Systems V, 83060L (11 October 2011); https://doi.org/10.1117/12.912264

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