Critical considerations of pupil alignment to achieve open-loop control of MEMS deformable mirror in nonlinear laser scanning fluorescence microscopy

Wei Sun; Yang Lu; Jason B. Stewart; Thomas G. Bifano; Charles P. Lin

doi:10.1117/12.909652

15 February 2012 Critical considerations of pupil alignment to achieve open-loop control of MEMS deformable mirror in nonlinear laser scanning fluorescence microscopy

Wei Sun, Yang Lu, Jason B. Stewart, Thomas G. Bifano, Charles P. Lin

Author Affiliations +

Proceedings Volume 8253, MEMS Adaptive Optics VI; 82530H (2012) https://doi.org/10.1117/12.909652
Event: SPIE MOEMS-MEMS, 2012, San Francisco, California, United States

Abstract

In this paper we present an alignment methodology for a non-linear laser scanning fluorescence microscopic imaging system integrated with a MEMS deformable mirror that is used to compensate microscope aberrations and improve sample image quality. The procedure uses an accurate open-loop control mechanism of the MEMS DM, a high resolution CMOS camera and a compact Shack-Hartmann wavefront sensor. The success of the indirect AO control method used by the microscope to compensate aberrations requires careful alignment of the optical system, specifically the DM conjugate planes in the scanning laser optical path. Considerations of this procedure are presented here, in addition to an assessment of the final accuracy of the alignment task is presented, by verifying the pupil conjugation and wavefront response. This method can also serve as a regular check-up of the system's performance and trouble-shoot for system misalignment.

Citation Download Citation

Wei Sun, Yang Lu, Jason B. Stewart, Thomas G. Bifano, and Charles P. Lin "Critical considerations of pupil alignment to achieve open-loop control of MEMS deformable mirror in nonlinear laser scanning fluorescence microscopy", Proc. SPIE 8253, MEMS Adaptive Optics VI, 82530H (15 February 2012); https://doi.org/10.1117/12.909652

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available