Wide-field near-infrared fluorescence endoscope for real-time in vivo imaging

Zhongyao Liu; Sharon J. Miller; Bishnu P. Joshi; Thomas D. Wang M.D.

doi:10.1117/12.906416

1 March 2012 Wide-field near-infrared fluorescence endoscope for real-time in vivo imaging

Zhongyao Liu, Sharon J. Miller, Bishnu P. Joshi, Thomas D. Wang M.D.

Proceedings Volume 8217, Endoscopic Microscopy VII; 82170G (2012) https://doi.org/10.1117/12.906416
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

A diode-pumped solid state laser is used to deliver excitation at λ_ex = 671 nm. The beam is expanded by a pair of relay lenses (f₁ = 30 and f₂ = 50 mm) to 3 mm diameter, filling the aperture of a fluid light cable that is coupled to a Hopkins II rigid endoscope. Near-infrared fluorescence images are collected by the endoscope and transmitted by another set of relay lenses onto a CCD detector that has dimensions of 8.7x6.9 mm² (1388x1040 pixels). A zoom lens system (F#1.6-16 aperture) with a tunable focal length (20-100 mm) magnifies the image to fill the dimensions of the CCD. A band pass filter allows fluorescence with spectral range λ_em = 696 to 736 nm to be collected. The system achieves a resolution of 9.8 μm and field-of-view of 3.6 mm at a distance of 2.5 mm between the distal end of the endoscope and the tissue. Images are collected at a rate of 10 frames per second. A filter wheel is incorporated into the handle of the instrument housing to rapidly switch between reflectance and fluorescence images. Cy5.5-labeled peptides were delivered through the 1 mm diameter instrument channel in the endoscope. Near-infrared fluorescence images demonstrated specific peptide binding to spontaneous adenomas that developed beginning at 2 months of age in a genetically-engineered mouse with mutation of one allele in the APC gene. This integrated methodology represents a powerful tool that can achieve real time detection of disease in the colon and other hollow organs.

Citation Download Citation

Zhongyao Liu, Sharon J. Miller, Bishnu P. Joshi, and Thomas D. Wang M.D. "Wide-field near-infrared fluorescence endoscope for real-time in vivo imaging", Proc. SPIE 8217, Endoscopic Microscopy VII, 82170G (1 March 2012); https://doi.org/10.1117/12.906416

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