Folate receptor targeted Type 1 photosensitizer bioconjugates for tumor visualization and phototherapy

Raghavan Rajagopalan; Amruta R. Poreddy; Amolkumar Karwa; Bethel Asmelash; Nicole E. Putnam; Lori Chinen; Maureen Nichols; J. Jeng Shieh; Richard B. Dorshow

doi:10.1117/12.875166

8 February 2011 Folate receptor targeted Type 1 photosensitizer bioconjugates for tumor visualization and phototherapy

Raghavan Rajagopalan, Amruta R. Poreddy, Amolkumar Karwa, Bethel Asmelash, Nicole E. Putnam, Lori Chinen, Maureen Nichols, J. Jeng Shieh, Richard B. Dorshow

Author Affiliations +

Proceedings Volume 7886, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX; 78860D (2011) https://doi.org/10.1117/12.875166
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Folate receptors are over expressed in many types of cancers, including, ovarian, breast, and cervical. In our continuing efforts toward the development of targeted Type 1 phototherapeutic agents, an azide-based Type 1 photosensitizer and a pyrzine-based fluorophore that absorb and emits in the visible region, and a dual diagnostic-therapeutic probe consisting of the fluorophore and the photosensitizer were prepared and independently conjugated to two folate receptor specific vectors: γ-carboxyl-modified folic acid and anti-human FOLR1 (folate receptor-1) antibody In vitro receptor binding study showed that all the conjugates had high (ca 1-7 nM) affinity to the folate receptor. Confocal microscopy images indicated that the pyrazine conjugates were selectively taken up by the folate receptor expressing ovarian cancer KB cells.

Citation Download Citation

Raghavan Rajagopalan, Amruta R. Poreddy, Amolkumar Karwa, Bethel Asmelash, Nicole E. Putnam, Lori Chinen, Maureen Nichols, J. Jeng Shieh, and Richard B. Dorshow "Folate receptor targeted Type 1 photosensitizer bioconjugates for tumor visualization and phototherapy", Proc. SPIE 7886, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX, 78860D (8 February 2011); https://doi.org/10.1117/12.875166

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available