Optofluidic single molecule flow proteometry

Nan Jing; Chao-Kai Chou; Mien-Chie Hung; Jun Kameoka

doi:10.1117/12.809442

5 March 2009 Optofluidic single molecule flow proteometry

Nan Jing, Chao-Kai Chou, Mien-Chie Hung, Jun Kameoka

Proceedings Volume 7185, Single Molecule Spectroscopy and Imaging II; 71850W (2009) https://doi.org/10.1117/12.809442
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A microfluidic single molecule fluorescence-based detection scheme is developed to identify target protein direct from cell lysate by using polyclonal antibody. Relative concentration of target protein in solution is determined by twodimensional (2D) photon burst analysis. Compared to conventional ensemble measurement assays, this microfluidic single molecule approach combines the advantages of higher sensitivity, fast processing time, small sample consumption and high resolution quantitative analysis.

Citation Download Citation

Nan Jing, Chao-Kai Chou, Mien-Chie Hung, and Jun Kameoka "Optofluidic single molecule flow proteometry", Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850W (5 March 2009); https://doi.org/10.1117/12.809442

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