Scattering performance analysis of reflectance photometer for quantifying gold labeled test strips

Lihua Huang; Youbao Zhang; Chengke Xie; Jianfeng Qu; Huijie Huang; Xiangzhao Wang

doi:10.1117/12.806529

27 January 2009 Scattering performance analysis of reflectance photometer for quantifying gold labeled test strips

Lihua Huang, Youbao Zhang, Chengke Xie, Jianfeng Qu, Huijie Huang, Xiangzhao Wang

Proceedings Volume 7156, 2008 International Conference on Optical Instruments and Technology: Optical Systems and Optoelectronic Instruments; 71561O (2009) https://doi.org/10.1117/12.806529
Event: International Conference of Optical Instrument and Technology, 2008, Beijing, China

Abstract

Gold labeled immunochromatography assay is widely used in many fields. Quantitative test can be realized by using a reflectance photometer. However, theoretical analysis of the strip and the photometer has seldom been reported. In this paper, the microstructure of immunochromatographic strip labeled by nanogold particles is analyzed with scanning electron microscope (SEM). Based on the SEM images of the strip, Mie's scattering theory is used to investigate the scattering behaviors of particles in the strip, and Lambert cosine law is applied to analyze the diffuse reflection of porous strip. Besides, a reflectance photometer for gold labeled test strip has been developed for fast quantification of immunochromatography assay in our group. Theoretical model is achieved by introducing the parameter of the developed reflectance photometer. The calculated scattering signal distribution is well consistent with that measured by the reflectance photometer.

Citation Download Citation

Lihua Huang, Youbao Zhang, Chengke Xie, Jianfeng Qu, Huijie Huang, and Xiangzhao Wang "Scattering performance analysis of reflectance photometer for quantifying gold labeled test strips", Proc. SPIE 7156, 2008 International Conference on Optical Instruments and Technology: Optical Systems and Optoelectronic Instruments, 71561O (27 January 2009); https://doi.org/10.1117/12.806529

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KEYWORDS

Particles

Gold

Scattering

Photometry

Reflectivity

Light scattering

Absorption

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