Fluorescence molecular-tomography reconstruction with a priori anatomical information

Lu Zhou; Birsen Yazici; Vasilis Ntziachristos

doi:10.1117/12.763269

13 February 2008 Fluorescence molecular-tomography reconstruction with a priori anatomical information

Lu Zhou, Birsen Yazici, Vasilis Ntziachristos

Proceedings Volume 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes; 68680O (2008) https://doi.org/10.1117/12.763269
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In this study, we combine a generalized Tikhonov regularization method with a priori anatomical information to reconstruct the concentration of fluorophores in mouse with Chronic Obstructive Pulmonary disease (COPD) from in vivo optical and Magnetic Resonance (MR) measurements. Generalized Tikhonov regularization incorporates a penalty term in the optimization formulation of the fluorescence molecular tomography (FMT) inverse problem. Our design involves two penalty terms to make use of a priori anatomical structural information from segmented MR images. The choice of the penalty terms guide the fluorophores in reconstructed image concentrates in the region where it is supposed to be and assure smooth flourophore distribution within tissue of same type and enhances the discontinuities between different tissue types. We compare our results with traditional Tikhanov regularization techniques in extensive simulations and demonstrate the performance our approach in vivo mouse data. The results show that the increased fluorophore concentration in the mouse lungs is consistent with an increased inflammatory response expected from the corresponding animal disease model.

Citation Download Citation

Lu Zhou, Birsen Yazici, and Vasilis Ntziachristos "Fluorescence molecular-tomography reconstruction with a priori anatomical information", Proc. SPIE 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes, 68680O (13 February 2008); https://doi.org/10.1117/12.763269

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