Internalization of fluorescent dextrans in the submandibular salivary glands of live animals: a study combining intravital two-photon microscopy and second harmonic generation

Andrius Masedunskas; Roberto Weigert

doi:10.1117/12.768051

15 February 2008 Internalization of fluorescent dextrans in the submandibular salivary glands of live animals: a study combining intravital two-photon microscopy and second harmonic generation

Andrius Masedunskas, Roberto Weigert

Author Affiliations +

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68601V (2008) https://doi.org/10.1117/12.768051
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Here we show that a combination of two-photon microscopy and second harmonic generation can be successfully used to study endocytosis in the submandibular salivary glands of live animals. First, we have characterized the threedimensional structure of the acini and the ducts forming the parenchyma of the excised glands by exciting various endogenous molecules, which highlight the shape of the cells and various components of the extracellular matrix. Next, by time-lapse imaging we show the dynamic distribution of fluorescent probes injected systemically. This was achieved by using a custom-made holder aimed to reduce the motion artifacts associated with the heartbeat and the respiration in the live animals. Finally, we show that fluorescent dextrans are internalized primarily by the supporting cells in the salivary glands, a characteristic shared by other secretory organs such as the pancreas.

Citation Download Citation

Andrius Masedunskas and Roberto Weigert "Internalization of fluorescent dextrans in the submandibular salivary glands of live animals: a study combining intravital two-photon microscopy and second harmonic generation", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601V (15 February 2008); https://doi.org/10.1117/12.768051

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