Extended focus Fourier domain optical coherence microscopy assists developmental biology

Martin L. Villiger; Manfred Beleut; Cathrin Brisken; Theo Lasser; Rainer A. Leitgeb

doi:10.1117/12.728370

11 July 2007 Extended focus Fourier domain optical coherence microscopy assists developmental biology

Martin L. Villiger, Manfred Beleut, Cathrin Brisken, Theo Lasser, Rainer A. Leitgeb

Proceedings Volume 6627, Optical Coherence Tomography and Coherence Techniques III; 66271H (2007) https://doi.org/10.1117/12.728370
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

We present a novel detection scheme for Fourier domain optical coherence microscopy (FDOCM). A Bessel-like interference pattern with a strong central lobe was created with an axicon lens. This pattern was then imaged by a telescopic system into the sample space to obtain a laterally highly confined illumination needle, extending over a long axial range. For increased efficiency, the detection occurs decoupled from the illumination, avoiding a double pass through the axicon. Nearly constant transverse resolution of ~1.5&mgr;m along a focal range of 200&mgr;m with a maximum sensitivity of 105dB was obtained. A broad bandwidth Ti:Sapphire laser allowed for an axial resolution of 3&mgr;m in air, providing the nearly isotropic resolution necessary to access the microstructure of biological tissues. Together with the speed- and sensitivity-advantage of FDOCT, this system can perform in vivo measurements in a minimally invasive way. Tomograms of the mouse mammary gland and the mouse follicle, recorded in vitro, revealed biologically relevant structural details. Images acquired with classical microscopy techniques, involving stained and fluorescent samples, validate these structures and emphasize the high contrast of the tomograms. It is comparable to the contrast achieved with classical techniques, but employing neither staining, labeling nor slicing of the samples, stressing the high potential of FDOCM for minimally invasive in vivo small animal imaging.

Citation Download Citation

Martin L. Villiger, Manfred Beleut, Cathrin Brisken, Theo Lasser, and Rainer A. Leitgeb "Extended focus Fourier domain optical coherence microscopy assists developmental biology", Proc. SPIE 6627, Optical Coherence Tomography and Coherence Techniques III, 66271H (11 July 2007); https://doi.org/10.1117/12.728370

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Mammary gland

Axicons

Tissues

In vivo imaging

Optical coherence tomography

Optical coherence microscopy

Green fluorescent protein

Show All Keywords

Keywords/Phrases

Search In:

Publication Years