Simultaneous excitation of multiple fluororophores with a compact femtosecond laser

Philippe Legros; Daniel Choquet; Stéphane Gueguen; Eric Mottay; Nelly Deguil

doi:10.1117/12.643774

23 February 2006 Simultaneous excitation of multiple fluororophores with a compact femtosecond laser

Philippe Legros, Daniel Choquet, Stéphane Gueguen, Eric Mottay, Nelly Deguil

Proceedings Volume 6089, Multiphoton Microscopy in the Biomedical Sciences VI; 60890U (2006) https://doi.org/10.1117/12.643774
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We report on a flexible multiphoton imaging system, suitable for simultaneous and efficient excitation of red (DsRed), yellow (YFP), green (GFP) and blue (DAPI) fluorophores. We used a simple, compact laser system, consisting of a 1 μm high energy diode-pumped oscillator and a tunable wavelength extension using a photonics crystal fibre. The combination of a near IR excitation wavelength, high energy per pulse for efficient three photon excitation and spectral extension for GFP excitation allows for high flexibility.We present experimental results of simultaneous and efficient imaging fluorophores couples from the UV to the red (DAPI-RFP, GFP-RFP).

Citation Download Citation

Philippe Legros, Daniel Choquet, Stéphane Gueguen, Eric Mottay, and Nelly Deguil "Simultaneous excitation of multiple fluororophores with a compact femtosecond laser", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 60890U (23 February 2006); https://doi.org/10.1117/12.643774

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