Dynamics of cells function on laser cell-chip system

Toshihiro Kushibiki; Tomoko Sano; Katsunori Ishii; Sachiko Yoshihashi-Suzuki; Kunio Awazu

doi:10.1117/12.646188

25 February 2006 Dynamics of cells function on laser cell-chip system

Toshihiro Kushibiki, Tomoko Sano, Katsunori Ishii, Sachiko Yoshihashi-Suzuki, Kunio Awazu

Proceedings Volume 6080, Advanced Biomedical and Clinical Diagnostic Systems IV; 60800B (2006) https://doi.org/10.1117/12.646188
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A new type of cell-cultivation system based on laser processing has been developed for the on-chip cultivation of living cells. We introduce a "laser cell-chip", on which migration of cells, such as stem cells, tumor cells or immunocompetent cells, can be observed. A sheet prepared from epoxy resin was processed by KrF excimer laser (248 nm, 1.6 J/cm²) for preparation of microgrooved surfaces with various groove width, spacing, and depth. A laser cell-chip can make kinetic studies of cell migration depending on the concentration gradient of a chemoattractant. In this study, megakaryocytes were used for the migration on a groove of laser cell-chip by the concentration gradient of the stromal cell derived factor 1 (SDF-1/CXCL12). SDF-1/CXCL12 plays an important and unique role in the regulation of stem/progenitor cell trafficking. A megakaryocyte was migrated on a groove of laser cell-chip depending on the optical concentration gradient of SDF-1/CXCL12. Since SDF-1/CXCL12-induced migration of mature megakaryocyte was known to increase the platelet production in the bone marrow extravascular space, the diagnosis of cell migration on laser cell-chip could provide a new strategy to potentially reconstitute hematopoiesis and avoid life-threatening hemorrhage after myelosuppression or bone marrow failure.

Citation Download Citation

Toshihiro Kushibiki, Tomoko Sano, Katsunori Ishii, Sachiko Yoshihashi-Suzuki, and Kunio Awazu "Dynamics of cells function on laser cell-chip system", Proc. SPIE 6080, Advanced Biomedical and Clinical Diagnostic Systems IV, 60800B (25 February 2006); https://doi.org/10.1117/12.646188

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Bone

Excimer lasers

Stem cells

Tumors

Tissues

Biological research

Laser systems engineering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years